28 Day Study Of Olive Oil For Hair Growth + 2 DIY Recipes!

In this article I’m going to show you exactly how you can start using olive oil for hair growth – Using a method that shows results within 28 days.

You’re going to learn about the scientific study carried out oleuropein (which is found in olive oil) which showed it was more effective than the leading topical hair loss product at regrowing hair.

Finally, I’ll show you how you can start using olive oil yourself to help reverse hair loss – and why there are other steps you need to do to, to give your hair the best chance of success.

What is Olive Oil?

Commonly used in the culinary arts as a frying oil or salad dressing, olive oil is an oil obtained from the olive crop.

This highly-valued tree crop, originating from the Mediterranean Basin, is made into oil through the process of pressing.

Beyond its use in the kitchen, olive oil has made a name for itself in the cosmetics and homeopathic industries and has been used medicinally for thousands of years.

From the banks of the Nile to the birthplace of democracy, olive oil has enjoyed a high status and widespread usage.

And now, as more individuals are turning to natural and organic cures for their ills, olive oil is being celebrated as a miracle of sorts—from its treatment of dry skin to its use as a hair growth stimulator.



Play with the slider below to see before and after.

Olive oil is also one of the main ingredients in Wild Growth hair Oil.

Olive Oil and Its Direct Impact on the Hair Growth Cycle

A recent study performed by Tong, Kim, and Park shows that oleuropein, a component found in the leaves of the olive tree, induces anagen hair growth in telogen mouse skin.

There are three phases within the hair growth cycle.

Phase 1, Anagen – This is the phase of active growth and rapid cell division.

Phase 2, Catagen – This phase is transitional and allows for the newly-grown hair sheath to properly attach to its root.

Phase 3, Telogen – This phase is known as the resting phase, where shedding typically occurs. During this phase of the cycle, healthy individuals will lose 25 – 100 hairs per day, on average.

Hair growth phase is important to understand the reasons behind hair loss

In individuals who suffer from alopecia, the anagen phase of the hair growth cycle significantly shortens over time.

As the length of the hair is proportional to the length of the anagen phase, the shorter the phase becomes, the shorter the hair which is produced.

Eventually, those with alopecia are left with hair which is too short to poke through the hair follicle, leading to thinning and baldness.

In this particular study, 24 mice were split into three groups of eight.

The backs of the mice were shaved at 8 weeks of age, and the telogen phase of the hair growth cycle was confirmed.

For 28 days, each mouse received a 200 μL application of either control, control + 0.4mg concentration of oleuropein, or control + 3mg concentration of Minoxidil.

The control consisted of 50% ethanol, 30% water, and 20% propylene glycol.

The mice were photographed on days 0, 7, 14, 21, and 28, and 10 random hairs from each mouse were measured on the same schedule.

Mice hair growth results using olive oil constituent

A comparison of hair growth in mice being treated with either A. a control; B. oleuropein; or C. minoxidil.

Oleuropein hair length mice

A comparison of the hair length (in cm) of mice treated with a control, oleuropein, or minoxidil.

Now, it’s no surprise that the hairs in the oleuropein-treated group were longer than those in the control group.

However, it is surprising that the oleuropein-treated group also had better results than the mice in the minoxidil-treated group.

What caused such drastic results?

Upregulation of Growth Factor Gene Expression

At the end of the 28-day study, skin samples of the mice were examined.

In the oleuropein-treated group, the mice had a significant increase in the mRNA levels in four separate growth factors (insulin-like growth factor – 1, hepatocyte growth factor, vascular endothelial growth factor, and keratocyte growth factor).

In fact, compared to mice treated with only the control, the mice in the oleuropein-treated group saw a 71% increase in dermal IGF-1 levels.

Each of the mentioned growth factors plays their own role in the growth and development of hair follicles. IGF-1, for example, is known to regulate cell proliferation which occurs rapidly during hair follicle formation.

There’s no doubt that the upregulation of growth factor gene expression played a significant role in the study’s results, but there’s another reason that treatment for hair growth proved to be so effective in the oleuropein-treated group of mice.

Stimulation of Wnt10b/β-Catenin Signaling Pathway

In a 2010 research study published in Developmental Cell, it was discovered that deletion of the β-catenin gene in dermal papilla cells of fully developed hair follicles led to the premature induction of the catagen phase in mice.

Further, the Wnt10b/β-catenin signaling pathway seems to play a role in the maintenance of the anagen phase.

In the oleuropein-treated group of mice mentioned in the previous study, researchers found that not only was there an increase in the mRNA levels of different growth factors, but oleuropein also led to the modulation of the Wnt10b/β-catenin pathway.

This pathway, proven to prolong the anagen phase, is largely responsible for the significant hair growth seen in the mice.

Scalp Inflammation and Olive Oil — A Proven Treatment

Certain forms of alopecia are caused by inflammation of the scalp.

This inflammation can be caused by a weakened immune system, allergies, or any other number of circumstances.

Whatever the source of the inflammation, the fact remains that chronic inflammation can be damaging to the hair follicles and induce premature hair thinning and loss.

As was shown in a 2008 randomized trial, the daily ingestion of virgin olive oil led to a decrease in circulating concentrations of interleukin-6, a known pro-inflammatory, and C-reactive protein, a responsive protein that indicates inflammation within the body.

But what makes olive oil such a powerful treatment option for inflammation, and are these results external as well as internal?

Oleocanthal, a phenolic compound within virgin olive oil, seems to be the underlying reason for olive oil’s effective treatment of inflammation and inflammatory diseases.

Phenolic compounds can be found within a number of plant-based foods, such as chili peppers, oregano, and sesame seeds.

Oleocanthal, however, is unique to virgin olive oil, and its various components are present within its very name (oleo – olive; canth – sting; and al – aldehyde).

This unique compound is responsible for the irritating sensation felt by most individuals in the back of the throat when olive oil is directly consumed.

Interestingly, this very attribute is believed to be felt most strongly in individuals who are particularly sensitive to the positive effects of oleocanthal.

And, while this phenolic compound is structurally dissimilar to Ibuprofen, the non-steroidal anti-inflammatory drug, both compounds inhibit the very same enzymes in the prostaglandin-biosynthesis pathway which triggers inflammation.

As a natural inhibitor of cyclooxygenase enzymes, there’s no wonder that oleocanthal plays such a large role in the reduction of inflammation.

Does this mean that the use of olive oil can reduce inflammation of the scalp for individuals with alopecia?

As previously discussed, oleocanthal reduces the circulating concentrations of interleukin-6 and other inflammation-causing enzymes.

This means that the anti-inflammatory effects are seen throughout the body, which makes oleocanthal a viable option for individuals looking to reduce scalp inflammation and irritation.

How to Use Olive Oil for the Treatment of Hair Loss

Olive oil is a commonly ingested oil, and ingestion of 2 tablespoons per day can be tolerated in those looking to supplement, though up to 1 liter per week has been safely used as part of the Mediterranean diet.

Another effective approach, however, is the application of olive oil directly to the scalp.

Whether through the use of homemade shampoo or the application of an all-natural scalp scrub, olive oil can be easily incorporated into your hair loss treatment routine.

DIY Olive Oil Shampoo

A particularly potent way to introduce olive oil into your regular hair care routine is by making your own homemade shampoo.

While the task may seem daunting, all you need is a cleansing agent + carrier oil + essential oil.

If you’re ready to get started, here’s a recipe which will reduce scalp inflammation and unclog blocked hair follicles.


  • Virgin olive oil (1 cup)
  • Evening primrose oil (1 teaspoon)
  • Apple cider vinegar (2 teaspoons)
  • Baking soda (1 teaspoon)


Combine all ingredients in the container or bottle of your choice.

Mix the ingredients thoroughly, and be sure to break up any baking soda clumps.

Wet your hair and scalp thoroughly in lukewarm water.

Lather the shampoo mixture onto your hair, and massage into the scalp for 2-4 minutes. After massaging, you may allow to sit for an additional 2 minutes if you’d like.

Rinse thoroughly.

Hair Benefits:

Evening primrose oil is an essential oil which boasts a number of hair growth benefits.

Its rich omega-6 composition makes it an effective treatment for hair loss, and its anti-inflammatory properties help to nourish the scalp and relieve any itching, inflammation, or general irritation.

The apple cider vinegar and baking soda combine to make a powerful, yet gentle, cleansing agent.

 Deep Cleansing Salt + Olive Oil Scrub

If you’re looking to cleanse your scalp while simultaneously enjoying olive oil’s many benefits, take a look at this 3-ingredient scalp scrub from hello glow as outlined below.


  • Sea salt (2 tablespoons)
  • Lemon juice (1-2 tablespoons)
  • Olive oil (1-2 tablespoons)


Add the salt, lemon juice, and olive oil into a container of your choice, and mix together well.

Wet your hair, and then massage the above mixture into your scalp for several minutes. Be sure to pay equal attention to all parts of your scalp.

Once down massaging, rinse the scrub thoroughly.

Hair Benefits:

The sea salt and olive oil in this simple scrub act as cleanser/moisturizer duo, which is a great combination for individuals with dandruff or hair product buildup.

In addition to the salt’s abrasive texture, the lemon juice breaks down the chemicals and pollutants which come into contact with your hair everyday.

Further, the olive oil’s fatty acid composition will protect your hair from breakage and keep your locks strong.

Are There Side Effects Associated with Olive Oil Supplementation?

While side effects associated with olive oil supplementation are few and far between, there are those individuals who may need to consult with their doctor prior to supplementation.

Such individuals include those with diabetes, low blood pressure, and those currently taking high blood pressure medications.

The consumption of olive oil can lower both blood sugar and blood pressure, and even with the consent of your physician, individuals with diabetes or low blood pressure should monitor their levels closely.

The best way to add olive oil to your diet is by using it as a dressing for salads and other foods.

Olive oil dressing

Contact dermatitis, or an allergic skin reaction, is rare. However, those with allergies may develop a skin rash similar to eczema and should cease supplementation immediately.

As always, women who are pregnant or nursing should consult with their obstetrician to ensure the safety of supplementation.


The studies have shown that oleuropein which is found in olive oil can significantly speed up hair -regrowth (in mice.)

It has proven to be more effective than the control, and the expensive (FDA approved) hair loss solution, minoxidil.

Since I personally tend to favour natural products I would definitely prefer to use olive oil rather than minoxidil.

Go here to learn about more natural alternatives to minoxidil.

However, this is just the first few steps to regrowing your hair.

The best way to rapidly reverse your hair loss is to fix the underlying cause of hair loss in the first place.

So, in conclusion, olive oil can be used for hair loss, but it will only help a very small amount.

The best course of action is to fix the underlying triggers to hair loss and then perhaps use olive oil to speed up hair re-growth.

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4 thoughts on “28 Day Study Of Olive Oil For Hair Growth + 2 DIY Recipes!”

  1. That’s pretty incredible to see that oleuropein beats minoxidil in a controlled study. I’m still not sure about using it directly on my scalp though, it could get a bit messy.

    • Hey olive oil is effective on the scalp but only a touch of it. I mean take a qtip and rub it lightly throughout scalp, if it makes you greasy…too much. Notice how good olive oil works on brows. Good to see it tested and proven.

    • Any will do, I used cheap grocery store brand, it’s helpful for sure, just not a lot or adversely it causes hair to fall out,at least for me.

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